The effect of blue and green light on human umbilical cord mesenchymal stem cells for promoting proliferation and wound healing.

Photobiomodulation (PBM) has been widely utilized in regenerative medicine, including dermatology, dentistry, and neurology. However, the optimal energy density of PBM for human umbilical cord mesenchymal stem cells (hUC-MSCs) remains underexplored, hindering its development and potential clinical application. This study aims to identify the optimal wavelength and irradiation fluence for promoting the proliferation of hUC-MSCs by comparing the effects of different wavelengths and irradiation fluences. Our results show that green light enhances the anti-inflammatory properties of hUC-MSCs, with the 76s being the most effective in inhibiting IL-6 and GM-CSF. Blue light with 38 s is more effective in promoting angiogenesis, significantly increasing the mRNA and protein secretion of VEGF, HGF, and FGF2 compared to the non-irradiated group. The peak secretion times varied, with VEGF and FGF2 peaking at 72 h and HGF at 24 h. RNA-Seq confirms the significant roles of blue and green light in inhibiting epithelial-mesenchymal transition and inflammation. In vitro co-culture models and conditioned media experiments validate these anti-inflammatory effects. These findings have important implications for accelerating the clinical application of stem cell therapies and provide new references for PBM use in hUC-MSCs.