重组ING4通过上皮-间质转化抑制SW579甲状腺癌细胞的迁移。
Recombinant ING4 suppresses the migration of SW579 thyroid cancer cells via epithelial to mesenchymal transition.
作者信息
Wang Chuan-Jiang, Yang Dong, Luo Ying-Wei
机构信息
Department of Vascular and Thyroid Surgery, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
出版信息
Exp Ther Med. 2015 Aug;10(2):603-607. doi: 10.3892/etm.2015.2515. Epub 2015 May 22.
Thyroid cancer is a common endocrine malignancy that has rapidly increased in global incidence. Inhibitor of growth 4 (ING4) has been identified in various types of carcinoma; however, to the best of our knowledge, no previous studies have investigated the effects of ING4 on thyroid cancer. In the present study, SW579 thyroid cancer cells were treated with recombinant ING4 protein, and the results confirmed that recombinant ING4 protein was able to reduce the rate of proliferation, increase the rate of apoptosis and inhibit the mobility of SW579 cells. These results were obtained using a colony formation, fluoroscein isothiocyanate/propidium iodide double staining and Transwell assays, respectively. Furthermore, in the western blot analysis assays, ING4 was demonstrated to inhibit the Wnt/β catenin signaling pathway and epithelial to mesenchymal transition (EMT). Therefore, the present study demonstrated the antitumor activities of recombinant ING4 and identified ING4 could inhibit EMT in thyroid cancer cell. However, additional studies are required to confirm these results in other cell types.
甲状腺癌是一种常见的内分泌恶性肿瘤，其全球发病率迅速上升。生长抑制因子4（ING4）已在多种类型的癌症中被发现；然而，据我们所知，以前没有研究调查过ING4对甲状腺癌的影响。在本研究中，用重组ING4蛋白处理SW579甲状腺癌细胞，结果证实重组ING4蛋白能够降低增殖率，提高凋亡率并抑制SW579细胞的迁移能力。这些结果分别通过集落形成、异硫氰酸荧光素/碘化丙啶双重染色和Transwell实验获得。此外，在蛋白质印迹分析实验中，ING4被证明可抑制Wnt/β连环蛋白信号通路和上皮-间质转化（EMT）。因此，本研究证明了重组ING4的抗肿瘤活性，并确定ING4可抑制甲状腺癌细胞中的EMT。然而，需要进一步的研究在其他细胞类型中证实这些结果。
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