环状RNA CDR1as通过靶向miR-876-5p/GNG7轴抑制胃癌转移
Circular RNA CDR1as Inhibits the Metastasis of Gastric Cancer through Targeting miR-876-5p/GNG7 Axis.
作者信息
Jiang Jiajia, Li Rong, Wang Junyi, Hou Jie, Qian Hui, Xu Wenrong
机构信息
Aoyang Institute of Cancer, Affiliated Aoyang Hospital of Jiangsu University, Zhangjiagang, Jiangsu 215600, China.
Zhenjiang Key Laboratory of High Technology Research on Exosomes Foundation and Transformation Application, Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013, China.
出版信息
Gastroenterol Res Pract. 2021 Jun 16;2021:5583029. doi: 10.1155/2021/5583029. eCollection 2021.
Circular RNA CDR1as has been demonstrated to participate in various cancer progressions as miRNA sponges. The exact underlying mechanisms of CDR1as on gastric cancer (GC) metastasis remain unknown. Here, we found that CDR1as knockdown facilitated GC cell migration and invasion while its overexpression inhibited the migration and invasion abilities of GC cells and . Moreover, epithelial-mesenchymal transition- (EMT-) associated proteins and MMP2 and MMP9 were downregulated by CDR1as. Bioinformatics analysis combined with dual-luciferase reporter gene assays, western blot, RT-qPCR analysis, and functional rescue experiments demonstrated that CDR1as served as a miR-876-5p sponge and upregulated the target gene GNG7 expression to suppress GC metastasis. In summary, our findings indicate that CDR1as suppresses GC metastasis through the CDR1as/miR-876-5p/GNG7 axis.
环状RNA CDR1as已被证明作为微小RNA海绵参与多种癌症进展。CDR1as在胃癌（GC）转移中的确切潜在机制尚不清楚。在此，我们发现敲低CDR1as促进GC细胞迁移和侵袭，而其过表达则抑制GC细胞的迁移和侵袭能力。此外，CDR1as下调上皮-间质转化（EMT）相关蛋白以及MMP2和MMP9。生物信息学分析结合双荧光素酶报告基因检测、蛋白质印迹、RT-qPCR分析和功能挽救实验表明，CDR1as作为miR-876-5p海绵，上调靶基因GNG7表达以抑制GC转移。总之，我们的研究结果表明，CDR1as通过CDR1as/miR-876-5p/GNG7轴抑制GC转移。
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