Effectiveness of mechanical cleaning, antibiotics, and induction heating on eradication of in mature biofilms.

AIMS

Here we used a mature seven-day biofilm model of exposed to antibiotics up to an additional seven days, to establish the effectiveness of either mechanical cleaning or antibiotics or non-contact induction heating, and which combinations could eradicate in mature biofilms.

METHODS

Mature biofilms of (ATCC 29213) were grown on titanium alloy (Ti6Al4V) coupons for seven days and were subjected to the following treatments or their combinations: antibiotics, mechanical cleaning, or heat shock by induction heating of 60°C for one minute. Experiments were repeated at least five times.

RESULTS

In the untreated biofilm, growth up to 1.8×10 colony-forming units (CFU)/cm was observed. Treatment with ciprofloxacin, flucloxacillin, vancomycin, cefuroxime, and amoxicillin all with rifampicin gave 6.0 log, 6.1 log, 1.4 log, 4.8 log, and 3.6 log reduction in CFU/cm, respectively. Mechanical cleaning alone resulted in 4.9 log reduction and induction heating in 7.3 log reduction. There was an additional effect of ciprofloxacin, flucloxacillin, and induction heating when used in combinations. There was no additional effect for mechanical cleaning. No bacterial growth could be detected after induction heating followed by seven days of ciprofloxacin with rifampicin.

CONCLUSION

Mechanical cleaning, antibiotics, and non-contact induction heating reduced the bacterial load of mature biofilms with approximately 5 log or more as a single treatment. The effect of mechanical cleaning on mature biofilms was limited when used in combination with antibiotics and/or induction heating.Cite this article:  2022;11(9):629-638.