用于选择性检测铁离子的简单荧光传感方法：活细胞成像与逻辑门功能
Simple Fluorescence Sensing Approach for Selective Detection of Fe Ions: Live-Cell Imaging and Logic Gate Functioning.
作者信息
Rasin Puthiyavalappil, Manakkadan Vipin, Vadakkedathu Palakkeezhillam Vishnunarayanan Namboothiri, Haribabu Jebiti, Echeverria Cesar, Sreekanth Anandaram
机构信息
Department of Chemistry, National Institute of Technology-Tiruchirappalli, 620015Tiruchirappalli, Tamil Nadu, India.
Facultad de Medicina, Universidad de Atacama, Los Carreras 1579, 1532502Copiapo, Chile.
出版信息
ACS Omega. 2022 Sep 12;7(37):33248-33257. doi: 10.1021/acsomega.2c03718. eCollection 2022 Sep 20.
A pyrene-based fluorescent chemosensor APSB [-(pyrene-1-ylmethylene) anthracen-2-amine] was designed and developed by a simple condensation reaction between pyrene carboxaldehyde and 2-aminoanthracene. The APSB fluorescent sensor selectively binds Fe in the presence of other metal ions. Apart from this, APSB shows high selectivity and sensitivity toward Fe ion detection. The detection limit for APSB was 1.95 nM, and the binding constant ( ) was obtained as 8.20 × 10 M in DMSO/water (95/5, v/v) medium. The fluorescence quantum yields for APSB and APSB-Fe were calculated as 0.035 and 0.573, respectively. The function of this fluorescent sensor APSB can be explained through the photo-induced electron transfer mechanism which was further proved by density functional theory studies. Finally, a live-cell image study of APSB in HeLa cells was also carried out to investigate the cell permeability of APSB and its efficiency for selective detection of Fe in living cells.
一种基于芘的荧光化学传感器APSB [-(芘-1-基亚甲基)蒽-2-胺] 通过芘甲醛与2-氨基蒽之间的简单缩合反应设计并开发而成。APSB荧光传感器在其他金属离子存在的情况下能选择性地结合铁。除此之外，APSB对铁离子检测表现出高选择性和高灵敏度。APSB的检测限为1.95 nM，在二甲基亚砜/水（95/5，v/v）介质中获得的结合常数（ ）为8.20×10 M。APSB和APSB-Fe的荧光量子产率分别计算为0.035和0.573。这种荧光传感器APSB的功能可通过光诱导电子转移机制来解释，密度泛函理论研究进一步证明了该机制。最后，还对APSB在HeLa细胞中的活细胞成像研究进行了，以研究APSB的细胞渗透性及其在活细胞中选择性检测铁的效率。
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