人类口腔黏膜作为临床应用中神经嵴干细胞的潜在有效来源。
Human Oral Mucosa as a Potentially Effective Source of Neural Crest Stem Cells for Clinical Practice.
机构信息
The Lundquist Institute for Biomedical Innovation, Torrance, CA 90502, USA.
Division of Oral & Maxillofacial Surgery and Hospital Dentistry, Department of Surgery Harbor UCLA Medical Center, Torrance, CA 90502, USA.
出版信息
Cells. 2023 Sep 6;12(18):2216. doi: 10.3390/cells12182216.
We report in this study on the isolation and expansion of neural crest stem cells (NCSCs) from the epithelium of oral mucosa (OM) using reagents that are GMP-certified and FDA-approved for clinical use. Characterization analysis showed that the levels of keratins , , , , , and -specific to OM epithelial cells-were significantly lower in the experimental NCSCs. While was decreased with no statistically significant difference, the earliest neural crest specifier genes , , , , , , and showed a trend in increased expression in NCSCs. In addition, proteins of , and were found to be greatly expressed, confirming NCSC multipotency. In conclusion, our study showed that the epithelium of OM contains NCSCs that can be isolated and expanded with clinical-grade reagents to supply the demand for multipotent cells required for clinical applications in regenerative medicine. Supported by Emmaus Medical Inc.
我们在这项研究中报告了使用经 GMP 认证并获得 FDA 批准可用于临床的试剂，从口腔黏膜（OM）上皮细胞中分离和扩增神经嵴干细胞（NCSC）的情况。特征分析表明，实验性 NCSC 中 OM 上皮细胞特有的角蛋白 、 、 、 、 和 -特异性明显降低。虽然 略有下降，但最早的神经嵴特异性基因 、 、 、 、 、 和 表达呈增加趋势。此外，还发现 、 和 的蛋白表达量大大增加，证实了 NCSC 的多能性。总之，我们的研究表明，OM 的上皮细胞中含有 NCSC，可以使用临床级别的试剂进行分离和扩增，以满足再生医学中临床应用所需的多能细胞的需求。这项研究得到了 Emmaus Medical Inc. 的支持。
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