哥伦比亚金迪奥地区急性腹泻儿童隐孢子虫和环孢子虫种类的分子鉴定

Molecular confirmation of Cryptosporidium and Cyclospora species in children with acute diarrhoea in Quindio region, Colombia.

作者信息

Triviño-Valencia Jessica, Nati-Castillo Alejandro, Cabeza Nancy Yhomara, Lora-Suarez Fabiana, Gómez-Marín Jorge

机构信息

Grupo GEPAMOL, Centro de Investigaciones Biomédicas, Facultad de Ciencias de la Salud, Universidad del Quindío, Armenia, Quindío, Colombia.

Grupo BIMSA, Departamento de Ciencias Básicas, Universidad Autónoma de Manizales, Universidad Autónoma de Manizales, Armenia, Quindío, Colombia.

出版信息

Gut Pathog. 2025 Mar 9;17(1):14. doi: 10.1186/s13099-025-00685-5.

DOI:10.1186/s13099-025-00685-5

PMID:40059177

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11892127/

Abstract

BACKGROUND

There are no reports with molecular confirmation of Cryptosporidium spp. and Cyclospora spp. in children consulting the emergency service due to diarrhoea in Colombia.

METHODS

A descriptive study was performed on 137 children who visited the Hospital San Juan de Dios Emergency Service in Armenia between April 1 and 31, 2022. Questionnaires and sampling were performed to identify parasites in the faecal samples. Fresh preparations were prepared with 1% iodine, and a modified Ziehl-Neelsen stain was used to identify pathogenic intestinal protozoa (Cryptosporidium spp. and Cyclospora spp.). PCR and sequencing of positive samples were performed to confirm infection.

RESULTS

The prevalence of Cryptosporidium spp. infection in children was 19,7%, and that of Cyclospora spp. was 10,9%. 59,2% of the children with cryptosporidiosis and 66,6% of the children with cyclosporiasis were hospitalized. PCR for Cryptosporidium spp. was positive in six of 28 (21%) samples and for Cyclospora in 11 of 15 (73%) samples. Cyclospora spp. SSU rRNA DNA sequences clustered 10 samples nearest to lineage A, two with lineage B, and one with lineage C.

CONCLUSIONS

Cryptosporidiosis and cyclosporiasis are common in children with acute diarrhoea when consulting emergency services, and their search should be performed systematically.

摘要

背景

在哥伦比亚，因腹泻到急诊就诊的儿童中，尚无隐孢子虫属和环孢子虫属分子确认的报告。

方法

对2022年4月1日至31日期间前往亚美尼亚圣胡安·迪奥斯医院急诊的137名儿童进行了描述性研究。通过问卷调查和采样来识别粪便样本中的寄生虫。用1%碘制备新鲜涂片，并使用改良齐尔-尼尔森染色法鉴定致病性肠道原生动物（隐孢子虫属和环孢子虫属）。对阳性样本进行PCR和测序以确认感染。

结果

儿童隐孢子虫属感染率为19.7%，环孢子虫属感染率为10.9%。隐孢子虫病患儿中有59.2%住院，环孢子虫病患儿中有66.6%住院。28份样本中有6份（21%）隐孢子虫属PCR检测呈阳性，15份样本中有11份（73%）环孢子虫PCR检测呈阳性。环孢子虫属小亚基核糖体RNA（SSU rRNA）DNA序列分析显示，10个样本最接近A系，2个样本属于B系，1个样本属于C系。