Relationship between PD-L1 expression and [F]FAPI versus [F]FDG uptake on PET/CT in lung cancer.

PURPOSE

To investigate the correlation between [F] labeled fibroblast activation protein inhibitor (FAPI) positron emission tomography (PET)/computed tomography (CT) uptake and programmed death ligand 1 (PD-L1) expression in lung cancer and evaluate the predictive value of [F]FAPI PET/CT for PD-L1 expression compared with [F]fluorodeoxyglucose ([F]FDG) PET/CT.

METHODS

This single-center retrospective study consecutively enrolled patients with pathologically confirmed lung cancer who underwent [F]FAPI and [F]FDG PET/CT scans within 2 weeks, with a minimum interval of 20 h. PD-L1 expression was assessed using immunohistochemistry and stratified into three groups. PET/CT uptake parameters included the maximum standard uptake value (SUV) in the biopsy tumor or mediastinal metastasis lymph nodes area and the mean SUV (SUV) of normal tissue (lung and blood). The ratios of SUV to the SUV for each normal tissue were denoted as the tumor-to-background ratios (TBR and TBR). All statistical analyses were conducted using IBM SPSS Statistics. Normality was assessed, and for non-normally distributed data, the Kruskal-Wallis and Mann-Whitney U tests were applied. Associations between variables were evaluated using Spearman's rank correlation. All tests were two-sided, with a P-value < 0.05 considered statistically significant.

RESULTS

Among the 75 cases included on the final analysis, the TBR and TBR derived from [F]FAPI PET/CT were significantly positively correlated with PD-L1 expression (r = 0.32, P < 0.01; r = 0.26, P < 0.05). Additionally, cases with high PD-L1 expression showed significantly higher [F]FAPI uptake values (mean TBR=36.16; mean TBR=10.75) compared with those with low PD-L1 expression (mean TBR=25.10; mean TBR=8.04). No statistically significant correlation was observed between [F]FDG uptake values and PD-L1 expression level. Receiver operating characteristic analysis identified TBR on [F]FAPI PET/CT with a cutoff value of 7.76 (area under the curve = 0.68, P < 0.01, sensitivity = 75%, and specificity = 53.49%) as a significant predictor of the level of PD-L1 expression.

CONCLUSION

[F]FAPI uptake was positively correlated with PD-L1 expression in lung cancer. The combination of [F]FAPI PET/CT and PD-L1 expression may offer a more comprehensive approach to assessing the response of lung cancer to immunotherapy.

TRIAL REGISTRATION

This study was approved by the Clinical Research Ethics Committee of our institution (institutional review board approval no. SDZLEC2021-112-02).