Determining the Multivalent Effects of d-Peptide-Based Radiotracers.

Dextrorotary (d) peptides, composed of d-amino acids, are hyper-resistant to proteolytic hydrolysis, making them valuable ligands with excellent stability for radiopharmaceutical development. Multimerization is a well-established strategy for enhancing the performance of l-peptide-based radiopharmaceuticals. However, the effect of multimerization on the fate of d-peptide-based radiopharmaceuticals remains largely unexplored. Here, we synthesized the d-peptide DPA, which targets PD-L1, along with its dimer (DP2) and trimer (DP3). PET/CT imaging and biodistribution studies were performed to delineate the pharmacokinetics and target interactions of [Ga]DPA, [Ga]DP2, and [Ga]DP3 in both normal and tumor-bearing mice. Our results revealed that tumor uptake and kidney retention increased with higher valency ([Ga]DP3 > [Ga]DP2 > [Ga]DPA). No significant differences were observed in the liver, heart, lung, spleen, intestine, or bone among the three radiotracers. Interestingly, a significant reduction of radioactivity in the bloodstream was detected for the [Ga]DP3-treated group compared to the other two groups. Data analysis revealed that chiral configuration of amino acids and the linking chemistry used in multimerization are the two dominant factors in the fate of d-peptide multimers. These findings indicate that d-peptide multimerization exerts a distinct influence on profiles compared to l-peptide multimerization. This study deepens our understanding of how mirror-imaged peptides/proteins interact with the living systems, paving the way for the development of radiopharmaceuticals that harness d-peptides as targeting moieties.