Shrivastava Siddhartha, Bera Tanmay, Roy Arnab, Singh Gajendra, Ramachandrarao P, Dash Debabrata
Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005, India.
Department of Metallurgy, Institute of Technology, Banaras Hindu University, Varanasi-221005, India.
Nanotechnology. 2007 May 4;18(22). doi: 10.1088/0957-4484/18/22/225103.
In the present study, we report the preparation of silver nanoparticles in the range of 10-15 nm with increased stability and enhanced anti-bacterial potency. The morphology of the nanoparticles was characterized by transmission electron microscopy. The antibacterial effect of silver nanoparticles used in this study was found to be far more potent than that described in the earlier reports. This effect was dose dependent and was more pronounced against gram-negative bacteria than gram-positive organisms. Although bacterial cell lysis could be one of the reasons for the observed antibacterial property, nanoparticles also modulated the phosphotyrosine profile of putative bacterial peptides, which could thus affect bacterial signal transduction and inhibit the growth of the organisms.
在本研究中,我们报告了制备出粒径在10至15纳米范围内、稳定性增强且抗菌效力提高的银纳米颗粒。通过透射电子显微镜对纳米颗粒的形态进行了表征。本研究中使用的银纳米颗粒的抗菌效果远强于早期报告中所描述的。这种效果具有剂量依赖性,对革兰氏阴性菌的作用比对革兰氏阳性菌更显著。虽然细菌细胞裂解可能是观察到的抗菌特性的原因之一,但纳米颗粒也调节了假定细菌肽的磷酸酪氨酸谱,从而可能影响细菌信号转导并抑制细菌生长。