使用原间隔序列工作台进行CRISPR应用的灵活引导RNA设计。 - Suppr

Flexible guide-RNA design for CRISPR applications using Protospacer Workbench.

作者信息

MacPherson Cameron Ross, Scherf Artur

机构信息

1] Biology of Host-Parasite Interactions Unit, Institut Pasteur, Paris, France. [2] CNRS, ERL 9195, Paris, France. [3] INSERM, Unit U1201, Paris, France.

出版信息

Nat Biotechnol. 2015 Aug;33(8):805-6. doi: 10.1038/nbt.3291. Epub 2015 Jun 29.

DOI:10.1038/nbt.3291

PMID:26121414

Abstract

摘要

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Flexible guide-RNA design for CRISPR applications using Protospacer Workbench.

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Int J Biol Sci. 2017 Nov 1;13(12):1470-1478. doi: 10.7150/ijbs.21312. eCollection 2017.

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Mol Biol (Mosk). 2020 Jan-Feb;54(1):29-50. doi: 10.31857/S0026898420010061.

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CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement.

Int J Mol Sci. 2022 Apr 18;23(8):4454. doi: 10.3390/ijms23084454.

本文引用的文献

Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation.

Nat Biotechnol. 2014 Dec;32(12):1262-7. doi: 10.1038/nbt.3026. Epub 2014 Sep 3.

sgRNAcas9: a software package for designing CRISPR sgRNA and evaluating potential off-target cleavage sites.

PLoS One. 2014 Jun 23;9(6):e100448. doi: 10.1371/journal.pone.0100448. eCollection 2014.

Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system.

Nat Biotechnol. 2014 Aug;32(8):819-21. doi: 10.1038/nbt.2925. Epub 2014 Jun 1.

E-CRISP: fast CRISPR target site identification.

Nat Methods. 2014 Feb;11(2):122-3. doi: 10.1038/nmeth.2812.

DNA targeting specificity of RNA-guided Cas9 nucleases.

Nat Biotechnol. 2013 Sep;31(9):827-32. doi: 10.1038/nbt.2647. Epub 2013 Jul 21.

Integrative genomics viewer.

Nat Biotechnol. 2011 Jan;29(1):24-6. doi: 10.1038/nbt.1754.

Zinc Finger Targeter (ZiFiT): an engineered zinc finger/target site design tool.

Nucleic Acids Res. 2007 Jul;35(Web Server issue):W599-605. doi: 10.1093/nar/gkm349. Epub 2007 May 25.

Flexible guide-RNA design for CRISPR applications using Protospacer Workbench.

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